Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40 (2025)

Dere, S., İspirli, H., Korkmaz, K., Tuncil, Y. E., Sagdic, O., Narbad, A., Ayyash, M., & Dertli, E. (2025). Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40. Scientific reports, 15(1), Article 11412. https://doi.org/10.1038/s41598-025-96324-9

Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40. / Dere, Sevda; İspirli, Hümeyra; Korkmaz, Kader et al.
In: Scientific reports, Vol. 15, No. 1, 11412, 12.2025.

Research output: Contribution to journal › Article › peer-review

Dere, S, İspirli, H, Korkmaz, K, Tuncil, YE, Sagdic, O, Narbad, A, Ayyash, M & Dertli, E 2025, 'Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40', Scientific reports, vol. 15, no. 1, 11412. https://doi.org/10.1038/s41598-025-96324-9

Dere S, İspirli H, Korkmaz K, Tuncil YE, Sagdic O, Narbad A et al. Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40. Scientific reports. 2025 Dec;15(1):11412. doi: 10.1038/s41598-025-96324-9

Dere, Sevda ; İspirli, Hümeyra ; Korkmaz, Kader et al. / Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40. In: Scientific reports. 2025 ; Vol. 15, No. 1.

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title = "Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40",

abstract = "Glucansucrases (GS) are functional enzymes for the production of distinct glucan and gluco-oligosaccharide structures with potential bioactive characteristics. In this study, an active glucansucrase (GS40) was identified within the genome of Leuconostoc mesenteroides YTU 40 that was further cloned, heterologously expressed and biochemically characterised. GS40 was capable of production of a branched dextran with ~ 20% (1→3)-α-D-Glc residues detected by1H and13C NMR analysis. Acceptor reactions of GS40 with maltose and cellobiose were performed and distinct maltose/cellobiose derivative acetylated gluco-oligosaccharides were produced detected by TLC, NMR and LC-MS analysis. The bioactive characteristics of branched dextran and gluco-oligosaccharides were tested by determination of their immune-modulatory roles in terms of triggering IL-12, IL-10, IL-4 and TNF-α cytokine levels. These findings can be important to unveil the role of glucansucrases for the production of gluco-oligosaccharides that can be used as functional ingredients for food and other industries.",

author = "Sevda Dere and H{\"u}meyra İspirli and Kader Korkmaz and Tuncil, {Yunus Emre} and Osman Sagdic and Arjan Narbad and Mutamed Ayyash and Enes Dertli",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2025.",

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T1 - Characterization and immune-modulatory roles of branched glucan and acetylated gluco-oligosaccharides produced by glucansucrase 40 from Leuconostoc mesenteroides YTU 40

AU - Dere, Sevda

AU - İspirli, Hümeyra

AU - Korkmaz, Kader

AU - Tuncil, Yunus Emre

AU - Sagdic, Osman

AU - Narbad, Arjan

AU - Ayyash, Mutamed

AU - Dertli, Enes

N1 - Publisher Copyright:© The Author(s) 2025.

PY - 2025/12

Y1 - 2025/12

N2 - Glucansucrases (GS) are functional enzymes for the production of distinct glucan and gluco-oligosaccharide structures with potential bioactive characteristics. In this study, an active glucansucrase (GS40) was identified within the genome of Leuconostoc mesenteroides YTU 40 that was further cloned, heterologously expressed and biochemically characterised. GS40 was capable of production of a branched dextran with ~ 20% (1→3)-α-D-Glc residues detected by1H and13C NMR analysis. Acceptor reactions of GS40 with maltose and cellobiose were performed and distinct maltose/cellobiose derivative acetylated gluco-oligosaccharides were produced detected by TLC, NMR and LC-MS analysis. The bioactive characteristics of branched dextran and gluco-oligosaccharides were tested by determination of their immune-modulatory roles in terms of triggering IL-12, IL-10, IL-4 and TNF-α cytokine levels. These findings can be important to unveil the role of glucansucrases for the production of gluco-oligosaccharides that can be used as functional ingredients for food and other industries.

AB - Glucansucrases (GS) are functional enzymes for the production of distinct glucan and gluco-oligosaccharide structures with potential bioactive characteristics. In this study, an active glucansucrase (GS40) was identified within the genome of Leuconostoc mesenteroides YTU 40 that was further cloned, heterologously expressed and biochemically characterised. GS40 was capable of production of a branched dextran with ~ 20% (1→3)-α-D-Glc residues detected by1H and13C NMR analysis. Acceptor reactions of GS40 with maltose and cellobiose were performed and distinct maltose/cellobiose derivative acetylated gluco-oligosaccharides were produced detected by TLC, NMR and LC-MS analysis. The bioactive characteristics of branched dextran and gluco-oligosaccharides were tested by determination of their immune-modulatory roles in terms of triggering IL-12, IL-10, IL-4 and TNF-α cytokine levels. These findings can be important to unveil the role of glucansucrases for the production of gluco-oligosaccharides that can be used as functional ingredients for food and other industries.

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